Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies.
Identifieur interne : 000082 ( Main/Exploration ); précédent : 000081; suivant : 000083Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies.
Auteurs : Benjamin Petre [Royaume-Uni, France] ; Diane G O. Saunders [Royaume-Uni] ; Jan Sklenar [Royaume-Uni] ; Cécile Lorrain [Royaume-Uni, France] ; Ksenia V. Krasileva [Royaume-Uni] ; Joe Win [Royaume-Uni] ; Sébastien Duplessis [France] ; Sophien Kamoun [Royaume-Uni]Source :
- PloS one [ 1932-6203 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Analyse de profil d'expression de gènes (MeSH), Basidiomycota (génétique), Basidiomycota (métabolisme), Basidiomycota (pathogénicité), Cadres ouverts de lecture (MeSH), Cartographie d'interactions entre protéines (MeSH), Chromatographie en phase liquide (MeSH), Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Maladies des plantes (microbiologie), Microscopie confocale (MeSH), Protéines fongiques (métabolisme), Protéines à fluorescence verte (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Simulation numérique (MeSH), Spectrométrie de masse en tandem (MeSH), Tabac (microbiologie), Triticum (microbiologie), Virulence (MeSH).
- MESH :
- génétique : Basidiomycota.
- microbiologie : Feuilles de plante, Maladies des plantes, Tabac, Triticum.
- métabolisme : ARN messager, Basidiomycota, Feuilles de plante, Protéines fongiques, Protéines à fluorescence verte.
- pathogénicité : Basidiomycota.
- Analyse de profil d'expression de gènes, Cadres ouverts de lecture, Cartographie d'interactions entre protéines, Chromatographie en phase liquide, Microscopie confocale, Régulation de l'expression des gènes végétaux, Simulation numérique, Spectrométrie de masse en tandem, Virulence.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (metabolism), Basidiomycota (pathogenicity), Chromatography, Liquid (MeSH), Computer Simulation (MeSH), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Green Fluorescent Proteins (metabolism), Microscopy, Confocal (MeSH), Open Reading Frames (MeSH), Plant Diseases (microbiology), Plant Leaves (metabolism), Plant Leaves (microbiology), Protein Interaction Mapping (MeSH), RNA, Messenger (metabolism), Tandem Mass Spectrometry (MeSH), Tobacco (microbiology), Triticum (microbiology), Virulence (MeSH).
- MESH :
- chemical , metabolism : Fungal Proteins, Green Fluorescent Proteins, RNA, Messenger.
- genetics : Basidiomycota.
- metabolism : Basidiomycota, Plant Leaves.
- microbiology : Plant Diseases, Plant Leaves, Tobacco, Triticum.
- pathogenicity : Basidiomycota.
- Chromatography, Liquid, Computer Simulation, Gene Expression Profiling, Gene Expression Regulation, Plant, Microscopy, Confocal, Open Reading Frames, Protein Interaction Mapping, Tandem Mass Spectrometry, Virulence.
Abstract
Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors.
DOI: 10.1371/journal.pone.0149035
PubMed: 26863009
PubMed Central: PMC4749346
Affiliations:
- France, Royaume-Uni
- Grand Est, Lorraine (région)
- Champenoux, Nancy, Vandœuvre-lès-Nancy
- Université de Lorraine
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Krasileva</name><affiliation wicri:level="1"><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Sainsbury Laboratory, Norwich Research Park, Norwich</wicri:regionArea><wicri:noRegion>Norwich</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Genome Analysis Centre, Norwich Research Park, Norwich, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Genome Analysis Centre, Norwich Research Park, Norwich</wicri:regionArea><wicri:noRegion>Norwich</wicri:noRegion></affiliation></author><author><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name><affiliation wicri:level="1"><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Sainsbury Laboratory, Norwich Research Park, Norwich</wicri:regionArea><wicri:noRegion>Norwich</wicri:noRegion></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement><settlement type="city" wicri:auto="agglo">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name><affiliation wicri:level="1"><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Sainsbury Laboratory, Norwich Research Park, Norwich</wicri:regionArea><wicri:noRegion>Norwich</wicri:noRegion></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>Basidiomycota (pathogenicity)</term><term>Chromatography, Liquid (MeSH)</term><term>Computer Simulation (MeSH)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Microscopy, Confocal (MeSH)</term><term>Open Reading Frames (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Protein Interaction Mapping (MeSH)</term><term>RNA, Messenger (metabolism)</term><term>Tandem Mass Spectrometry (MeSH)</term><term>Tobacco (microbiology)</term><term>Triticum (microbiology)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Basidiomycota (génétique)</term><term>Basidiomycota (métabolisme)</term><term>Basidiomycota (pathogénicité)</term><term>Cadres ouverts de lecture (MeSH)</term><term>Cartographie d'interactions entre protéines (MeSH)</term><term>Chromatographie en phase liquide (MeSH)</term><term>Feuilles de plante (microbiologie)</term><term>Feuilles de plante (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Microscopie confocale (MeSH)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Simulation numérique (MeSH)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Tabac (microbiologie)</term><term>Triticum (microbiologie)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Green Fluorescent Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Tabac</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Tobacco</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Basidiomycota</term><term>Feuilles de plante</term><term>Protéines fongiques</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Liquid</term><term>Computer Simulation</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Microscopy, Confocal</term><term>Open Reading Frames</term><term>Protein Interaction Mapping</term><term>Tandem Mass Spectrometry</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Cadres ouverts de lecture</term><term>Cartographie d'interactions entre protéines</term><term>Chromatographie en phase liquide</term><term>Microscopie confocale</term><term>Régulation de l'expression des gènes végétaux</term><term>Simulation numérique</term><term>Spectrométrie de masse en tandem</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26863009</PMID><DateCompleted><Year>2016</Year><Month>07</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>22</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>2</Issue><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies.</ArticleTitle><Pagination><MedlinePgn>e0149035</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0149035</ELocationID><Abstract><AbstractText>Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petre</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saunders</LastName><ForeName>Diane G O</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Genome Analysis Centre, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The John Innes Centre, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sklenar</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lorrain</LastName><ForeName>Cécile</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krasileva</LastName><ForeName>Ksenia V</ForeName><Initials>KV</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Genome Analysis Centre, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Win</LastName><ForeName>Joe</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duplessis</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kamoun</LastName><ForeName>Sophien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002853" MajorTopicYN="N">Chromatography, Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003198" MajorTopicYN="N">Computer Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025941" MajorTopicYN="N">Protein Interaction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>01</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>7</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId 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Kamoun</name><name sortKey="Krasileva, Ksenia V" sort="Krasileva, Ksenia V" uniqKey="Krasileva K" first="Ksenia V" last="Krasileva">Ksenia V. Krasileva</name><name sortKey="Krasileva, Ksenia V" sort="Krasileva, Ksenia V" uniqKey="Krasileva K" first="Ksenia V" last="Krasileva">Ksenia V. Krasileva</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><name sortKey="Sklenar, Jan" sort="Sklenar, Jan" uniqKey="Sklenar J" first="Jan" last="Sklenar">Jan Sklenar</name><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name></country><country name="France"><region name="Grand Est"><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></region><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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